Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Type I IFN-producing CD4 Valpha14i NKT cells facilitate priming of IL-10-producing CD8 T cells by hepatocytes.

doi: 10.4049/jimmunol.178.4.2083

Figure Lengend Snippet: FIGURE 6. The cytokine response of OT-I CD8 T cells primed by HC with or without NKT cell help. A, OT-I CD8 T cells (105/well) were cocul- tured with GalCer- and SIINFEKL peptide-pulsed HC (104/well) with () or without () NKT cells (4 104/well). Cells were harvested after a 72-h culture, incubated for 4 h with ionomycin (750 ng/ml) and PMA (50 ng/ml), washed, and tested for IL-10 secretion in a 3-h capture assay. Cells were labeled with a PE-conjugated anti-IL-10 mAb and an anti-CD8 mAb. Data from a representative of five independent experiments are shown. B, OT-I CD8 T cells (105/well) were cocultured for 72 h with GalCer-/ SIINFEKL peptide-pulsed HC (104/well) with () or without () NKT cells (4 104/well). CD8 T blasts were harvested, purified, and restimu- lated in vitro for 48 h with peptide-pulsed DC. IFN-, IL-2, and IL-10 in 48-h supernatants of these secondary cultures were determined by ELISA. Mean values of triplicates (SEM) of a representative of four independent experiments are shown.

Article Snippet: IL-10-producing CD8 T cells were identified by a cytokine capture assay (catalog no. 130-090-489; Miltenyi Biotec) combined with surface staining with the allophycocyanin-conjugated anti-CD8 mAb 53-6.7 (catalog no. 553035; BD Biosciences).

Techniques: Incubation, Labeling, In Vitro, Enzyme-linked Immunosorbent Assay

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Active Fraction Combination from Liuwei Dihuang Decoction (LW-AFC) Alleviated the LPS-Induced Long-Term Potentiation Impairment and Glial Cells Activation in Hippocampus of Mice by Modulating Immune Responses

doi: 10.1155/2019/3040972

Figure Lengend Snippet: Effect of LW-AFC on cytokine secretion in the (a) hippocampus and (b) plasma of LPS-treated mice. ∗∗∗ p < 0.001, ∗∗ p < 0.01, and ∗ p < 0.05 vs. con and Student's t -test; ### p < 0.001, ## p < 0.01, and # p < 0.05 vs . mod, one-way ANOVA, and Dunnett's test, mean ± SD, n = 5–8. A, IL-1 β ; B, IL-6; C, MCP-1; D, G-CSF; E, IL-10; F, TNF- α ; G, IL-6; H, IFN γ ; I, IL-12; J, IL-10. con, control; mod, model; INDO, indomethacin.

Article Snippet: The secretion of TNF- α in the cell supernatant was determined using an AlphaLISA mouse TNF- α Kit (PerkinElmer, Waltham, MA, USA) according to the manufacturer's protocols using an EnVisionTM 2104 instrument (PerkinElmer, Waltham, MA, USA).

Techniques: Clinical Proteomics, Control

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Active Fraction Combination from Liuwei Dihuang Decoction (LW-AFC) Alleviated the LPS-Induced Long-Term Potentiation Impairment and Glial Cells Activation in Hippocampus of Mice by Modulating Immune Responses

doi: 10.1155/2019/3040972

Figure Lengend Snippet: Effect of compounds from LW-AFC on cell viability and secretion of TNF- α in LPS-stimulated and non-LPS-stimulated BV-2 cells: (a) the cell viability of non-LPS-stimulated BV-2 cells; (b) the cell viability of LPS-stimulated BV-2 cells; (c) secretion of TNF- α in non-LPS-stimulated BV-2 cells; (d) secretion of TNF- α in LPS-stimulated BV-2 cells. ∗∗∗ p < 0.001 and ∗∗ p < 0.01 vs . con and Student's t -test; ### p < 0.001, ## p < 0.01, and # p < 0.05, vs . mod, one-way ANOVA, and Dunnett's test. Mean ± SD n = 3. con, control; mod, model; INDO, indomethacin; DEX, dexamethasone.

Article Snippet: The secretion of TNF- α in the cell supernatant was determined using an AlphaLISA mouse TNF- α Kit (PerkinElmer, Waltham, MA, USA) according to the manufacturer's protocols using an EnVisionTM 2104 instrument (PerkinElmer, Waltham, MA, USA).

Techniques: Control